Abstract

This study investigates the growth and micropropagation of the rare moss Podperaea krylovii, under in vitro conditions. The effects of different growth media, plant growth regulators, and sugars were tested to optimize conditions for efficient propagation and to gain a better understanding of the species’ biology and the cultivation requirements of this species. The results indicate that the KNOP medium is most effective for the propagation of P. krylovii, with prostrate-oriented explants having a higher index of multiplication compared to upright-oriented explants. The addition of auxin had no significant effect on species multiplication and development in comparison to auxin-free media. Conversely, cytokinin inhibited shoot formation as well as protonemal development, suggesting that its use individually was not suitable for the species’ development under axenic conditions. The sugars tested showed no positive effect on new shoot formation, suggesting that no exogenous carbon source is required and that this species keeps autotrophy in axenic cultures. These results provide a basis for future research on the propagation and cultivation of P. krylovii and emphasize the need for specific cultivation protocols. The in vitro multiplicated moss was successfully acclimatised and propagated ex vitro in a xenic environment. This enables further successful ex situ conservation of the species as well as commercial use as aquatic plants, for example in aquaria.

Addeddate

2025-05-23 19:55:48

Bhl_virtual_titleid

210922

Bhl_virtual_volume

v.58 (2025)

Call number

10_3897_natureconservation_58_150920

Call-number

10_3897_natureconservation_58_150920

Foldoutcount

0

Genre

article

Identifier

advancesconserv58jadr

Identifier-ark

ark:/13960/s2h2q3zk7c3

Identifier-bib

10_3897_natureconservation_58_150920

Identifier-doi

10.3897/natureconservation.58.150920

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

26

Page_number_module_version

1.0.5

Page_range

289-309

Pages

21

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Nature Conservation 58

Year

2025

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